子痫前期患者血清miR-372-3p、miR-222-3p水平对不良妊娠结局的预测价值

Predictive value of serum mir-372-3p and mir-222-3p levels in patients with preeclampsia on adverse pregnancy outcomes

摘要:

目的 探讨血清微小RNA-372-3p(miR-372-3p)、微小RNA-222-3p(miR-222-3p)水平预测子痫前期(PE)患者不良妊娠结局的价值。方法 选取2021年2月至2024年2月于西北妇女儿童医院就诊的110例PE患者为PE组，依据其病情分为轻度PE组41例和重度PE组69例；选取同期于该院产检的108例正常孕妇为对照组，记录各组妊娠结局及相关临床资料。采用qRT-PCR法检测血清miR-372-3p、miR-222-3p水平；采用Logistic回归分析筛选影响因素；绘制ROC曲线分析指标预测妊娠结局的价值。结果 PE组血清miR-372-3p、miR-222-3p水平高于对照组(P<0.05)。重度PE组血清miR-372-3p、miR-222-3p水平高于轻度PE组(P<0.05)。结局不良组24 h尿蛋白、收缩压、舒张压及血清miR-372-3p、miR-222-3p水平高于结局良好组(P<0.05)。miR-372-3p、miR-222-3p为PE患者不良妊娠结局的独立危险因素，二者联合预测PE患者不良妊娠结局的AUC高于miR-372-3p、miR-222-3p单独预测(Z=2.804、2.186,P<0.05)。结论 PE患者血清miR-372-3p、miR-222-3p水平异常升高，与疾病进展和不良妊娠结局相关，二者联合后可有效预测PE患者不良妊娠结局。

Abstract:

Objective Toexplorethevalueofserum microRNA-372-3p (miR-372-3p)and microRNA-222-3p (miR-222-3p)levelsinpredictingadversepregnancyoutcomesinpatientswithpreeclampsia(PE).Methods Atotalof110 patientswithPEtreatedinNorthwestWomen'sandChildren'sHospitalfromFebruary2021toFebruary2024wereincluded asthePEgroup,andwerefurthergroupedintoamildPEgroup (n=41)andaseverePEgroup (n=69)basedontheir diseaseseverity.Meanwhile,108normalpregnantwomenwhounderwentprenatalcheckupswereselectedasthecontrol group.Thepregnancyoutcomeandrelatedclinicaldatawererecorded.Quantitativereal-timepolymerasechainreaction (qRT-PCR)wasusedtodetectserum miR-372-3pand miR-222-3plevels.Logisticregressionanalysiswasperformedto screenforinfluencingfactors.Receiveroperatorcharacteristic (ROC)curvewasplottedtoexplorethevalueofserum miR-372-3pandmiR-222-3plevelsinpredictingpregnancyoutcomesinPEpatients.Results TheserumlevelsofmiR-372-3p andmiR-222-3pinthePEgroupwerehigherthanthoseinthecontrolgroup(P<0.05).TheserumlevelsofmiR-372-3p andmiR-222-3pintheseverePEgroupwerehigherthanthoseinthemildPEgroup(P<0.05).Thelevelsof24hurinary protein,systolicbloodpressure,diastolicbloodpressureandserum miR-372-3pandmiR-222-3pinthepooroutcomegroup werehigherthanthoseinthegoodoutcomegroup(P<0.05).miR-372-3pandmiR-222-3pwereindependentriskfactorsfor adversepregnancyoutcomesinPEpatients.Theareaunderthecurve(AUC)ofthecombinedpredictionof miR-372-3pand miR-222-3p for adverse pregnancy outcomes in PE patients was higher than that of their single prediction (Z=2.804,2.186,P<0.05).Conclusion Theserum levelsof miR-372-3pand miR-222-3pin patients with PE are abnormallyelevated.Theelevatedlevelsofthetwoindicatorsareassociatedwithdiseaseprogressionandadversepregnancy outcomes.ThecombinationofthetwoindicatorscaneffectivelypredictadversepregnancyoutcomesinPEpatients.