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Volume 21,2026 Issue 3
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2026, Doi: 10.3969/j.issn.1674-2257.2026.03.001
Abstract:
Objective To investigate the impact of Echinococcus granulosus (Eg) infection on M2 macrophages in mice and to explore the underlying mechanisms based on the interleukin (IL)-4/Janus kinase 1 (JAK1)/signal transducer and activator of transcription 6 (STAT6) signaling pathway. Methods An Eg infection model was established in 40 specific pathogen-free (SPF) male BALB/c mice via intraperitoneal injection of a protoscolex suspension. The infected mice were randomly divided into 4-, 8-, 12-, and 24-week groups (n = 10 per group) according to the duration of infection. Additionally, 40 healthy mice served as the control group and received intraperitoneal injection of an equal volume of normal saline. General condition of mice in each group was observed at the end of the infection period. Hepatic histopathological changes were examined by hematoxylin-eosin (HE) staining. The proportions of M1-polarized macrophages (F4/80+ CD86+) and M2-polarized macrophages (F4/80+ CD206+) in peritoneal lavage fluid were determined by flow cytometry. The mRNA expression levels of the M1 polarization markers [tumor necrosis factor-α (TNF-α), and IL-6] and M2 polarization markers [transforming growth factor-β (TGF-β), IL-4, and IL-10] in mouse liver tissues were measured by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The expression levels of IL-4/JAK1/STAT6 signaling pathway-related proteins (IL-4, p-JAK1, STAT6, and p-STAT6) in mouse liver tissues were detected by Western blotting. Results Compared with the control group, hepatocytes in all Eg-infected groups exhibited disordered arrangement, edema, and inflammatory cell infiltration. However, these pathological manifestations were alleviated at 24 weeks. The proportion of F4/80+ CD86+ M1 macrophages in the peritoneal lavage fluid was increased in all Eg-infected groups compared with the control group, and TNF-α mRNA expression was increased in the 8-week group (P < 0.05). The proportion of F4/80+ CD206+ M2 macrophages was increased in the 12-week and 24-week groups (P<0.05). The expression levels of M2 polarization markers and IL-4/JAK1/STAT6 signaling pathway-related proteins were increased in the 8-, 12-, and 24-week groups (P<0.05). Conclusion Echinococcus granulosus infection promotes the polarization of macrophages towards the M2 phenotype in mice through the IL-4/JAK1/STAT6 signaling pathway.
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Liu Yanjiang; Chen Yixuan; Yuan Jiaxin; Pan Kejian
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.002
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Objective To investigate the protective effect of Edaravone (EDA) against radiation-induced intestinal injury (RIII) and its underlying molecular mechanism. Methods C57BL/6J mice were randomly divided into a control group, an irradiation (IR) group (RIII model), an IR+EDA group (RIII model with EDA intervention), and an IR+NAC group [positive control, RIII model with N-acetylcysteine (NAC) treatment], with 11 mice per group. The effects of EDA on mouse survival rate, body weight, intestinal histopathology, expression of the cell proliferation marker Ki67, and oxidative stress indicators were observed. Additionally, a radiation injury model was established in rat small intestinal crypt epithelial IEC-6 cells for the detection of changes in cell viability, reactive oxygen species (ROS), glutathione (GSH), solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), and phosphorylated histone H2AX (γ-H2AX). Transcriptomic analysis, real-time fluorescence quantitative polymerase chain reaction (qPCR), and Western blotting were performed to analyze the regulatory role of the phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT)/nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway. Results Compared with the IR group, the IR+EDA group showed improved survival rate (P<0.05), slower weight loss, and increased intestinal villus length, crypt depth, and Ki67-positive cell count (P<0.05). In IEC-6 cells, compared with the IR group, the IR+EDA group exhibited reduced oxidative stress-related ROS levels (P<0.001), increased GSH content (P<0.05), and upregulated protein and mRNA levels of ferroptosis markers SLC7A11 and GPX4 (P<0.05). Additionally, in the IR+EDA group, both p-PI3K/PI3K and p-AKT/AKT levels were lower than those in the IR group, while the protein expression level and mRNA level of Nrf2 were higher than those in the IR group (P<0.05). Conclusion Edaravone may inhibit ferroptosis-mediated radiation-induced intestinal injury by regulating the PI3K-AKT/Nrf2 signaling pathway.
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Li Li; Gao Zhenyan; Meng Yajun
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.003
Abstract:
Objective To investigate the role of microRNA-128-3p (miR-128-3p) in apical periodontitis and its molecular mechanism via regulation of transforming growth factor beta receptor 2 (TGFBR2). Methods 1) In vitro experiments: An inflammatory model was established in human oral keratinocyte cells (HOK-16B) by stimulation with Porphyromonas gingivalis lipopolysaccharide (P.gingivalis LPS). The cells were divided into Control group, Model group, miR-128-3p mimics group, miR-128-3p NC (negative control) group, and OE-TGFBR2 group (TGFBR2-overexpressing adenovirus transfection group). Flow cytometry was used to detect apoptosis, and dual-luciferase reporter assay was performed to validate the targeted regulatory relationship between miR-128-3p and TGFBR2. Western blotting was used to detect the expression levels of TGFBR2/Smad signaling pathway-related proteins, tight junction protein ZO-1, and TGF-β1. 2) In vivo experiments: An apical periodontitis model was established in Sprague-Dawley (SD) rats. The rats were divided into Control group, Model group, miR-128-3p agomir group, miR-128-3p NC group, and miR-128-3p agomir+OE-TGFBR2 group, with 12 rats in each group. Micro-computed tomography (micro-CT) was used to evaluate microstructural parameters of periapical bone tissue, including bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), and trabecular separation (Tb.Sp). Hematoxylin-eosin staining was used to observe histopathological damage of periapical tissues. Serum levels of inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, IL-33] were measured by enzyme-linked immunosorbent assay (ELISA). The expression of tartrate-resistant acid phosphatase (TRAP) in periapical tissues was detected by immunohistochemistry. Results In the cell experiments, compared with the Model group, the miR-128-3p mimics group showed a decreased apoptosis rate (P<0.001), as well as decreased protein expression levels of p-Smad, ZO-1, TGFBR2, and TGF-β1 (P<0.001). Dual-luciferase reporter assay showed that compared with the miR-128-3p NC group, the expression of WT-TGFBR2 in the miR-128-3p mimics group was decreased (P<0.001). In the animal experiments, compared with the Model group, the miR-128-3p agomir group showed increased BMD, BV/TV, and Tb.N, and decreased Tb.Sp (P<0.001); reduced histopathological damage (P<0.001); and decreased levels of inflammatory factors (IL-6, IL-1β, TNF-α, IL-33) as well as a reduced TRAP-positive area (P<0.001). Conclusion miR-128-3p alleviates inflammatory response and bone destruction by targeting and inhibiting TGFBR2, thereby playing a protective role in apical periodontitis.
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Zhu Yingting; Wang Yuan; Luo Ming; Chen Jie; Luo Zhihua
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.004
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Objective To investigate the effect of long non-coding RNA potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 (LncRNA KCNQ1OT1) on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced microglial injury by regulating the microRNA (miR)-183-5p/autophagy-related protein 5 (ATG5) axis. Methods Human HMC3 microglia cultured in vitro were randomly assigned into Con group, OGD/R group, sh-NC group, sh-LncRNA KCNQ1OT1 group, sh-LncRNA KCNQ1OT1 + anti-NC group, and sh-LncRNA KCNQ1OT1 + anti-miR-183-5p group. The mRNA expression of LncRNA KCNQ1OT1, miR-183-5p, and ATG5 were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The dual-luciferase reporter assay was used to verify the regulatory relationship between LncRNA KCNQ1OT1 and miR-183-5p, and between miR-183-5p and ATG5. Cell counting kit-8 (CCK-8), flow cytometry, and Western blotting were used to assess the cell proliferation and apoptosis, and the expression of related proteins. Western blotting was used to detect the levels of ATG5 and autophagy-related proteins. Results Compared with the OGD/R or sh-NC groups, the sh-LncRNA KCNQ1OT1 group exhibited decreased levels of LncRNA KCNQ1OT1, ATG5 mRNA and ATG5 protein, apoptosis rate, cleaved caspase-3, Bax, and LC3 II/I ratio, along with increased levels of miR-183-5p, cell survival rate, proliferating cell nuclear antigen (PCNA), Bcl-2, and P62 proteins (P<0.05). Compared with the sh-LncRNA KCNQ1OT1 or sh-LncRNA KCNQ1OT1+ anti-NC groups, the sh-LncRNA KCNQ1OT1 + anti-miR-183-5p group showed no significant change in LncRNA KCNQ1OT1 in cells (P >0.05), but displayed increased levels of ATG5 mRNA and ATG5 protein, apoptosis rate, cleaved caspase-3, Bax, and LC3 II/I ratio, alongside decreased levels of miR-183-5p, cell survival rate, PCNA, Bcl-2, and P62 proteins (P<0.05). LncRNA KCNQ1OT1 negatively regulated miR-183-5p through targeted binding, while miR-183-5p subsequently exerted negative regulation on ATG5 (P<0.05). Conclusion Downregulation of LncRNA KCNQ1OT1 can inhibit OGD/R-induced autophagy and apoptosis in HMC3 microglia by regulating miR-183-5p/ATG5 axis, thereby suppressing cell injury.
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Li Xin; Bao Lishuai; Cai Zencun; Wei Jiaojun; Zhao Long
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.005
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Objective To investigate the synergistic effect of Rhein and piezoelectric stimulation in composite hydrogel microspheres on regulating macrophage M1/M2 polarization, providing a theoretical and experimental basis for inflammatory disease treatment. Methods Composite hydrogel microspheres encapsulating Rhein-loaded liposomes and barium titanate nanoparticles were prepared via microfluidics and ultraviolet irradiation. Their morphology, composition, drug loading, piezoelectric properties, biocompatibility, and ability to induce M1 to M2 macrophage polarization were systematically evaluated. Results Composite hydrogel microspheres with both sustained Rhein release and piezoelectric properties were successfully prepared, showing good biocompatibility. Under ultrasound stimulation, they significantly upregulated the M2 macrophage marker CD206 compared to other groups. Conclusion The anti-inflammatory drug Rhein and piezoelectric stimulation can synergistically regulate the polarization of inflammatory M1 macrophages toward anti-inflammatory M2 macrophages.
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2026, Doi: 10.3969/j.issn.1674-2257.2026.03.006
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Objective To investigate the effect of fraxetin on inducing apoptosis in multiple myeloma (MM) cells via the p38 mitogen-activated protein kinase (MAPK) signaling pathway and its underlying mechanism. Methods The effect of fraxetin on the proliferation of MM cells was detected by the cell counting kit-8 (CCK-8) assay. Network pharmacology was adopted to predict the targets of both the disease MM and the drug fraxetin, and common target genes of the two were identified by analyzing the intersection targets. Protein-protein interaction (PPI) network construction, Gene Ontology (GO) functional enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed on the common targets. JC-1 and Annexin V-APC/7-AAD staining combined with flow cytometry were used to detect the mitochondrial membrane potential and apoptosis in MM cells induced by fraxetin. The underlying mechanism of fraxetin's anti-proliferative effect on MM cells was further explored by Western blotting. Results After fraxetin treatment, the proliferation of MM.1S, MM.1R, and RPMI8266 MM cells was inhibited, with the 24-hour half-maximal inhibitory concentration (IC50) values of 39.97, 34.70, and 62.34 μmol/L, respectively. PPI network analysis revealed that core intersection targets between MM and fraxetin involved EGFR, HSP90AA1, MAPK14, AKT1, SRC, PTK2, etc. The GO function and KEGG pathway enrichment analyses suggested that the intersection target genes were primarily associated with apoptosis and the MAPK signaling pathway, etc. JC-1 and Annexin V-APC/7-AAD staining results indicated that fraxetin could induce changes in mitochondrial membrane potential and apoptosis in MM cells. Western blot analysis showed that after fraxetin treatment, the expression levels of apoptosis-related proteins cleaved poly (ADP-ribose) polymerase (PARP) and cleaved Caspase-3 were upregulated in the cells (P<0.05). The expression level of the pro-apoptotic protein Bax increased, while the levels of the anti-apoptotic proteins Bcl-2 and Bcl-xL decreased (P<0.05). Furthermore, the expression level of phosphorylated p38 (p-p38), a key protein in the MAPK signaling pathway, was elevated (P<0.05). Conclusion The p38 MAPK signaling pathway mediates the apoptosis of cells induced by fraxetin via the mitochondrial pathway, thereby exerting the activity of inhibiting the proliferation of MM cells.
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Acupuncture and Moxibustion Improves Cognitive Function in MCAO Rats via BDNF/TrkB Signaling Pathway
Xiao Yaping; Yuan Hongli; Xu Jianming; Li Xiujuan
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.007
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Objective To explore the effects of acupuncture and moxibustion for warming Yang, invigorating Spleen and replenishing Qi on cognitive function in rats with post-stroke cognitive impairment (PSCI). Methods Sixty healthy male Sprague-Dawley (SD) rats were randomly divided into 5 groups via a random number table: blank control, model [PSCI model established via left middle cerebral artery occlusion (MCAO)], warm acupuncture (PSCI model + acupuncture + moxibustion), acupuncture alone (PSCI model + acupuncture), and moxibustion alone (PSCI model + moxibustion) groups, with 12 rats per group. All interventions lasted for 21 days. The composition and structural characteristics of the gut microbiota were analyzed using 16S rRNA sequencing, cognitive function by Morris water maze (MWM), brain-derived neurotrophic factor (BDNF) and tropomyosin-related kinase B (TrkB) protein expression in hippocampal/colon tissues by Western blotting, neurological deficit by Zea-Longa score, and neuronal damage in the hippocampal CA1 and dentate gyrus (DG) regions by pathological sectioning. Results Compared with the blank control group, the model group showed disrupted gut microbiota structure, decreased microbial diversity and evenness (P < 0.05), increased neurological deficit scores (P<0.05), prolonged escape latency in the MWM (P<0.001), reduced target quadrant exploration time, fewer platform crossings, and decreased hippocampal neuron count (P<0.05), as well as downregulated protein expression of BDNF and TrkB in the hippocampus (P<0.01). However, compared with the model group, the warm acupuncture group reversed the above indicators (P<0.05), and the improvements across all measured parameters were superior to those in the acupuncture alone and the moxibustion alone groups (P < 0.05). Conclusion Acupuncture and moxibustion for warming Yang, invigorating Spleen and replenishing Qi can improve cognitive function in PSCI rats by restructuring the gut microbiota balance and activating the BDNF/TrkB signaling pathway via the gut-brain axis.
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2026, Doi: 10.3969/j.issn.1674-2257.2026.03.008
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Objective To investigate the effect of microRNA-383-3p (miR-383-3p) overexpression on ferroptosis in renal ischemia-reperfusion injury (IRI)-induced acute kidney injury (AKI). Methods C57BL/6J mice were randomly divided into five groups (n = 5 per group): Sham, model (IRI), ferrostatin-1 (Fer-1), empty vector (NC), and miR-383-3p overexpression (miR-383-3p+). Except for the Sham group, all other groups underwent IRI modeling by bilateral renal pedicle clamping. Four days before modeling, corresponding lentiviral solutions were administered via tail vein injection. Hematoxylin and eosin (HE) staining was used to observe the glomerular and tubulointerstitial pathological changes in each group. A microplate colorimetric assay was employed to measure the levels of creatinine (Cr), blood urea nitrogen (BUN), glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), malondialdehyde (MDA), and ferrous iron (Fe2?). The protein expression level of glutathione peroxidase 4 (GPX4) in the kidney was detected by immunohistochemistry. And the protein expression levels of acyl-CoA synthetase long-chain family member 4 (ACSL4), solute carrier family 7 member 11 (SLC7A11), and heme oxygenase-1 (HO-1) in the tissues were determined by Western blotting. Results Compared with the Sham group, the IRI group showed increased levels of Cr, BUN, GSH, MDA, HO-1, and ACSL4 (P<0.05), and decreased levels of GSH-Px, Fe2?, SOD, GPX4, and SLC7A11 (P<0.05). Compared with the IRI group, the miR-383-3p+ group showed decreased levels of Cr, BUN, MDA, HO-1, and ACSL4 (P<0.05), and increased levels of GSH, GSH-Px, Fe2?, SOD, GPX4, and SLC7A11 (P<0.05). Conclusion Overexpression of miR-383-3p alleviates IRI-induced acute kidney injury, and its protective mechanism is closely associated with the inhibition of renal tubular ferroptosis.
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Wang Fengbo; Fu Qian; Cai Xing; Jiang Yuan
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.009
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Objective To investigate the effects and mechanism of repetitive transcranial magnetic stimulation (rTMS) on Beclin-1 expression in the hippocampus and cognitive function in rats with diabetic encephalopathy (DE). Methods Sixty-six adult healthy Sprague-Dawley (SD) rats were randomly divided into a sham-operated group (n=24) and a DE model group (n=42) using a random number table. DE model rats were fed a high-sugar, high-fat diet and received intraperitoneal injections of streptozotocin. Three days after injection, random blood glucose measured from the tail vein was ≥16.7 mmol/L, and the result was confirmed no less than twice within two consecutive weeks. At week 4 post-injection, Morris water maze (MWM) testing confirmed impaired cognitive function in the model group compared to the sham-operated group (P<0.05), indicating successful DE model establishment. The successfully modeled DE rats were then randomly subdivided into a non-intervention group (n=24) and an rTMS group (n=18). Subsequently, 6 rats each from the sham-operated and non-intervention groups were randomly sacrificed for Beclin-1 immunohistochemical detection in the hippocampal CA1 region. The rTMS group underwent rTMS intervention for 21 consecutive days. Six rats from each group were randomly selected for Beclin-1 detection on days 7 and 14 of the intervention. Beclin-1 expression was detected on day 21 of the intervention after the final MWM test. Results Beclin-1 expression in the DE model group was lower than that in the sham-operated group (P<0.05), and was higher in the rTMS group than that in the non-intervention group (P<0.05). In the MWM test, the rTMS group showed greater improvements compared to the non-intervention group in mean escape latency, time spent in the original platform quadrant, and number of platform crossings (P<0.05). Conclusion rTMS can upregulate Beclin-1 expression in the hippocampal CA1 region of diabetic encephalopathy rats, modulate the autophagy mechanism, and ameliorate cognitive impairment.
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Li Xiaoyan; Li Qianqian; Liu Xuejie
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.010
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Objective To investigate the effects of sirtuin 2 (SIRT2) overexpression on the malignant biological behaviors of endometrioid adenocarcinoma cells and the underlying mechanism. Methods A subcutaneous xenograft tumor model was established in female non-obese diabetic/severe combined immunodeficient (NOD-SCID) mice. The mice were divided into a control group (inoculated with human endometrial cancer Ishikawa cells) and a SIRT2 overexpression group (inoculated with Ishikawa cells overexpressing SIRT2). Tumor volume was measured every 3 d for 21 d consecutively, and the tumor growth curve was plotted. Ishikawa cells were also divided into a control group (untransfected), a negative control (NC) group (transfected with negative control plasmid), and a SIRT2 overexpression group (transfected with SIRT2 plasmid). Western blotting was performed to detect the expression levels of programmed cell death protein 1 (PD-1), programmed death 1 ligand-1 (PD-L1), protein kinase B (AKT), phosphorylated AKT (p-AKT), mechanistic target of rapamycin (mTOR), and phosphorylated mTOR (p-mTOR) in tumor tissues and cells. Cell apoptosis was assessed by flow cytometry. Cell migration was evaluated by Transwell assay. Results In vivo experiments in mice revealed that compared with the control group, SIRT2 overexpression led to increased tumor volume (P<0.01) and upregulated expression levels of PD-1 and PD-L1 in tumor tissues (P<0.01). In vitro cell experiments demonstrated that compared with the control and NC groups, the SIRT2 overexpression group exhibited a decreased apoptosis rate and enhanced migratory capacity (P<0.05), while no significant differences were observed between the NC and control groups (P>0.05). Compared with the control and NC groups, the SIRT2 overexpression group showed upregulated PD-1 and PD-L1 expression (P<0.05). No significant changes were observed in total AKT and mTOR protein levels (P>0.05), whereas p-AKT and p-mTOR levels were markedly upregulated (P<0.01). Conclusion SIRT2 overexpression can inhibit apoptosis and promote migration of human endometrial cancer Ishikawa cells, potentially through the activation of the AKT-mTOR signaling pathway, which may induce the expression of PD-1/PD-L1.
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Zhang Wen; Gan Yunong; Zeng Qin; Zeng Jiuzhi; Xu Yisha; Zhang Siyuan; Dai Min; Guo Wei
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.011
Abstract:
Objective To explore the differences in diversity and composition of seminal microbiota between men with abnormal and normal semen quality. Methods A total of 19 male subjects recruited at the Reproductive Medicine Center, Sichuan Provincial Maternity and Child Health Care Hospital from January to March 2022 were enrolled. Based on semen quality criteria, they were divided into a normal group (n=5) and an abnormal group (n=14). Semen microbiota was analyzed using 16S rDNA high-throughput sequencing, followed by bioinformatics and statistical analysis. Results Significant differences in microbial species composition and relative abundance were observed between the two groups. The Shannon and Chao1 indices of the semen microbiota in the abnormal group were higher than those in the normal group (P<0.05). At the phylum level, the relative abundances of Actinobacteria, Chloroflexi, and Acidobacteria were higher in the abnormal group than in the normal group (P<0.05). At the genus level, the relative abundance of Staphylococcus was higher in the normal group than in the abnormal group (P<0.05). Compared with the normal group, the abnormal group showed higher relative abundances of Ezakiella, Fenollaria timonensis, Peptoniphilus lacrimalis, Gaiella, Anaerococcus, Solirubrobacterales, Mobiluncus, Escherichia-Shigella, Bifidobacterium, Varibaculum, Chlamydia trachomatis, and Anaerococcus mediterraneensis (P < 0.05), and lower relative abundances of Staphylococcus, Staphylococcaceae, Bacillales, Alloprevotella, and Rothia (P<0.05). Conclusion There are significant differences in the diversity and composition of seminal microbiota between men with abnormal semen quality and those with normal semen quality. Ezakiella and Staphylococcus may serve as potential microbial biomarkers to distinguish abnormal semen parameters.
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Peng Jifang; Wang Xiuqin; Shao Yongfeng; Liu Changhong; Gao Chunhong
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.012
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Objective To evaluate the intervention effect of traditional Chinese medicine (TCM) diet therapy based on syndrome differentiation combined with auricular acupressure on constipation in elderly patients after coronary artery bypass grafting (CABG). Methods A randomized controlled design was used to select elderly patients who underwent CABG in the Department of Cardiovascular Surgery at the First Affiliated Hospital with Nanjing Medical University from January to June 2025. Ultimately, 98 patients completed the study and were included in the intention-to-treat analysis. The control group (n=48) received routine postoperative treatment and nursing care. On the basis of routine nursing, the experimental group (n=50) received auricular acupressure initiated within 24 h after surgery, supplemented with TCM diet therapy based on syndrome differentiation upon gastrointestinal function recovery. The intervention continued until postoperative day 7 or discharge. TCM constipation symptom (major and secondary) scores, postoperative constipation incidence, nursing satisfaction, and treatment compliance were compared between the two groups. Results After intervention, the experimental group showed greater improvement than the control group in the scores of both major TCM constipation symptoms (time to first defecation, defecation interval, defecation speed, and stool characteristics) and secondary TCM constipation symptoms (tenesmus, abdominal distension, and abdominal pain) (P<0.05). Compared to the control group, the experimental group demonstrated earlier time to first flatus and time to first defecation, a lower incidence of constipation, and higher nursing satisfaction (P<0.05). There was no statistically significant difference in treatment compliance between the two groups (P>0.05). Conclusion The combination of TCM diet therapy based on syndrome differentiation and auricular acupressure can effectively alleviate postoperative constipation symptoms, promote gastrointestinal function recovery, reduce the incidence of constipation, and improve nursing satisfaction in elderly patients after CABG surgery. This integrated approach is safe and feasible, without imposing additional physiological or financial burden on patients.
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Zhou Menghui; Li Lunlan; Tong Jiwen; Lu Yiyu; Sun Yexiang; Hu Delin; Hu Yi
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.013
Abstract:
Objective To explore the clinical effects of the Manchester Pain Management Model (MPMM) combined with empowerment education on alleviating negative emotions and promoting scar rehabilitation in burn patients. Methods A total of 84 patients in the Department of Burns of the First Affiliated Hospital of Anhui Medical University from June 2024 to January 2025 were selected using convenience sampling method, and were divided into a control group (n=42, receiving routine treatment and nursing care) and an intervention group (n=42, receiving a comprehensive intervention of MPMM combined with empowerment education in addition to routine care) based on their admission time. Patients were evaluated using the Numeric Rating Scale (NRS), the Simplified Coping Style Questionnaire (SCSQ), the Self-Rating Anxiety Scale (SAS), the Self-Rating Depression Scale (SDS), and the Vancouver Scar Scale (VSS). Results After the intervention, the intervention group had a NRS score at 0 h after functional exercise of (3.50±1.11), an SAS standard score of (48.26 ± 10.49), an SDS standard score of (45.73±9.15), an SCSQ negative coping dimension score of (10.90±3.27), and a VSS total score of (7.04±0.93), all of which were lower than those in the control group; whereas the SCSQ positive coping dimension score in the intervention group (27.77±9.48) was higher than that in the control group. All differences were statistically significant (P<0.05). Conclusion The Manchester Pain Management Model combined with empowerment education can reduce pain during functional exercise, alleviate negative emotions, improve treatment compliance, and promote functional rehabilitation in burn patients.
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Liu Luyao; Kang Wenwen; Peng Aili; Peng Wenjing
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.014
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Objective To investigate the influencing factors of concurrent cognitive impairment in patients with severe burns and to construct a nomogram prediction model. Methods This study adopted a retrospective cohort design. A total of 241 patients with severe burns admitted to a hospital from July 2021 to April 2024 were selected as the training set, and another 31 patients with severe burns admitted to the same hospital from May 2024 to December 2024 served as the external validation set (test set). Clinical data within 24 h of admission were collected as predictors and the Montreal Cognitive Assessment (MoCA) was adopted to assess whether cognitive impairment (CI) occurred after the patients' conditions stabilized. Based on the assessment results, the training set was divided into the training set-CI group (n=84) and the training set-non-CI group (n=157), while the test set was divided into the test set-CI group (n = 16) and the test set-non-CI group (n = 15). Univariate and multivariate logistic regression analyses were performed on the training set data to identify independent risk factors for CI in patients with severe burns, and a visualized nomogram prediction model was established. Results Univariate analysis showed that the training set-CI group had higher values than the training set-non-CI group in terms of age, percentage of total body surface area burned (%TBSA), length of hospital stay, duration of surgery, rates of mechanical ventilation use, incidence of intraoperative hypotension and hypoxemia, rates of postoperative return to intensive care unit (ICU), postoperative infection rates, and the proportions of patients with severe perioperative pain, adverse psychological status, history of alcohol consumption, and multiple surgeries (P<0.05). Multivariate logistic regression analysis indicated that age, %TBSA, length of hospital stay, duration of surgery, and educational level were all independent risk factors for CI in patients with severe burns (P<0.05). The nomogram model constructed based on these factors performed well in both the training and test sets. Conclusion The nomogram prediction model constructed in this study can effectively predict the risk of concomitant cognitive impairment in patients with severe burns, with good accuracy and clinical applicability, which aids in the early identification of high-risk populations and guides intervention.
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Yi Simeng; Li Zhixinyi; Peng Xian; Wang Shida
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.015
Abstract:
Objective To investigate the composition and interactions of bacterial and viral communities in dental plaque from patients with moderate to severe caries, and to elucidate the potential role of bacteriophages in microbial dysbiosis. Methods Ten patients with moderate to severe dental caries treated at the Department of Endodontics, West China Hospital of Stomatology, Sichuan University from September 2023 to February 2025 were recruited. Using a self-controlled design, dental plaque samples were collected from the caries-affected surfaces group and non-caries-affected surfaces group. Metagenomic and viromic sequencing were performed, and bacterial-phage interaction networks were constructed based on diversity, differential and cooccurrence network analyses. Results Significant differences in β-diversity and community composition were observed between the two groups for both bacterial and viral communities (P < 0.05). In the caries-affected surfaces group, the abundance of bacteria such as Agrobacterium sp. RAC06 and bacteriophages such as Bacillus phage BCPST was significantly increased. Network analysis identified Streptococcus, Actinomyces, and Veillonella as key nodes. The proportion of lytic phages was higher in the caries-affected surfaces group, accompanied by evidence of cross-host infection. Conclusion Microbial dysbiosis in caries-associated dental plaque is characterized by alterations in community structure and function. Bacteriophages may contribute to the onset and progression of dental caries by modulating the bacterial community.
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Wang Xijia; Wang Xiaoying; Liu Universe; Meng Zhe
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.016
Abstract:
Objective To explore the inhibitory effect and mechanism of early intervention of sodium-glucose cotransporter 2 inhibitor (SGLT2i) on ventricular remodeling in diabetic patients with ST-segment elevation myocardial infarction (STEMI). Methods A retrospective analysis was conducted on 57 diabetic patients with STEMI who received early SGLT2i intervention at the First Affiliated Hospital of Zhengzhou University from October 2023 to October 2024 (intervention group). Another 57 diabetic patients with STEMI receiving conventional treatment in the same hospital during the same period were included as the control group. Blood glucose levels, echocardiographic parameters, and serum myocardial injury markers before and after treatment, as well as the incidence of adverse reactions were compared between the two groups. Results After treatment, both groups showed reductions in fasting plasma glucose (FPG), postprandial 2-hour blood glucose (2hPG), glycated hemoglobin A1c (HbA1c), cardiac troponin T (cTnT), B-type natriuretic peptide (BNP), and creatine kinase-myocardial band (CK-MB) levels compared to baseline (P<0.05). And the levels of the above indicators in the intervention group after treatment were lower than those in the control group (P<0.05). After treatment, the levels of left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), left ventricular end-diastolic dimension (LVEDD), and interventricular septal thickness (IVST) in both groups were all lower than those before treatment (P<0.05), while the level of left ventricular ejection fraction (LVEF) was higher than that before treatment (P<0.05). The levels of the LVEDV, LVESV, LVEDD, IVST in the intervention group after treatment were lower than those in the control group (P<0.05), while the level of LVEF was higher than that in the control group (P<0.05). There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion Early SGLT2i intervention significantly improves cardiac function and inhibits ventricular remodeling in diabetic patients with STEMI, potentially through alleviating myocardial injury, with a favorable safety profile.
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2026, Doi: 10.3969/j.issn.1674-2257.2026.03.017
Abstract:
Objective To investigate the efficacy of nape acupuncture combined with Tongdu Tiaoshen acupuncture in treating patients with dysphagia in the convalescent stage of cerebral infarction. Methods A total of 90 patients with cerebral infarction in the convalescent stage complicated with dysphagia admitted to the Second Affiliated Hospital of Anhui University of Chinese Medicine from January 2023 to December 2024 were enrolled as study subjects. They were randomly assigned into an experimental group (n=45) and a control group (n=45) using a random number table. The control group received conventional swallowing function training plus Tongdu Tiaoshen acupuncture, while the experimental group received additional nape acupuncture alongside the regimen given to the control group. Both groups underwent a 2-week treatment course. Before and after treatment, swallowing function, swallowing-related quality of life, and oral feeding ability were assessed using the Eating Assessment Tool-10 (EAT-10), the Swallowing Quality of Life Questionnaire (SWAL-QOL), and the Functional Oral Intake Scale (FOIS), respectively. Follow-up evaluations at the first and third months after discharge included EAT-10 reassessment, satisfaction survey, and recording of complications. Results After treatment, both groups showed decreased EAT-10 scores and increased SWAL-QOL and FOIS scores compared with baseline (P<0.05), and the experimental group outperformed the control group in all three measures (P<0.05). At both the 1- and 3-month follow-ups, the experimental group maintained lower EAT-10 scores than the control group (P < 0.05). No statistically significant differences were observed between the two groups in the incidence of complications, rehospitalization rate, or patient satisfaction (P>0.05). Conclusion Nape acupuncture combined with Tongdu Tiaoshen acupuncture significantly improves swallowing function in patients with dysphagia after cerebral infarction, with sustained long-term efficacy.
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Xilina Yi Maimiti; Wang Lingling
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.018
Abstract:
Objective To explore the diagnostic value of the combined detection of fibroblast growth factor 21 (FGF21), galectin-3 (Gal-3), and interleukin-33 (IL-33) for human brucellosis. Methods A total of 100 patients with brucellosis admitted to the People's Hospital of Xinjiang Uygur Autonomous Region from January 2023 to January 2025 were enrolled as the brucellosis group (including 41 cases in the acute phase and 59 in the chronic phase). Additionally, 62 healthy individuals undergoing physical examinations during the same period were selected as the control group. Serum levels of FGF21, Gal-3, and IL-33 were measured using enzyme-linked immunosorbent assay (ELISA). Patients in the acute phase received a course of treatment (≥ 6 weeks) with doxycycline combined with rifampin, supplemented by antipyretic and analgesic therapy, nutritional support, and immunomodulatory therapy. Patients in the chronic phase received three courses of treatment with the aforementioned combined antibiotic regimen, with the addition of a quinolone-based regimen or a triple-antibiotic regimen if necessary. Results There were statistically significant differences in liver function indicators alanine aminotransferase (ALT), aspartate aminotransferase (AST), and direct bilirubin (DBil), as well as blood routine indicators white blood cell count (WBC), platelet count (PLT), neutrophil count (NEUT), and hemoglobin (HGB) among patients in the control group, chronic brucellosis group, and acute brucellosis group (P<0.05). Serum levels of FGF21, Gal-3, and IL-33 in the brucellosis group were higher than those in the control group, both before and after treatment (P<0.05). Pre-treatment serum levels of FGF21, Gal-3, and IL-33 in both acute and chronic phase patients were higher than post-treatment levels (P<0.05), and the levels in the acute phase patients were higher than those in the chronic phase patients (P<0.05). Elevated levels of FGF21, Gal-3, and IL-33 were identified as risk factors for the occurrence of brucellosis (P<0.05). The combined detection of FGF21, Gal-3, and IL-33 achieved an area under the curve (AUC) of 0.978 for diagnosing brucellosis, which was higher than that of any single marker alone (P<0.05). Conclusion Serum levels of FGF21, Gal-3, and IL-33 are significantly elevated in patients with brucellosis. The combined detection of the three biomarkers demonstrates high diagnostic value for human brucellosis.
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2026, Doi: 10.3969/j.issn.1674-2257.2026.03.019
Abstract:
Objective To explore prognostic factors for mechanical thrombectomy (MT) in patients with acute ischemic stroke (AIS) due to anterior circulation large vessel occlusion (LVO) and to construct a predictive model for poor outcomes. Methods A total of 229 patients with AIS due to anterior circulation LVO who underwent MT at Nanchong Central Hospital from July 2020 to November 2023 were included. Based on the 90-day modified Rankin Scale (mRS) scores, patients were categorized into a good outcome group (mRS≤2, n=81) and a poor outcome group (mRS≥3, n=148). Clinical data including demographic and clinical characteristics, blood biochemical indices, and procedural metrics were collected. Results Univariate analysis revealed that the following parameters differed between the two groups (P<0.05): history of atrial fibrillation, age, preoperative National Institutes of Health Stroke Scale (NIHSS) score, Alberta Stroke Program Early CT Score (ASPECTS), blood glucose, platelet count, aspartate aminotransferase, activated partial thromboplastin time, postprocedural intracranial hemorrhage, and puncture-to-recanalization time (PTR). Multivariate logistic regression analysis identified the preoperative NIHSS score (OR=1.215, 95%CI: 1.120-1.318) and PTR (OR=1.013, 95%CI: 1.004-1.021) as independent risk factors for the clinical outcome after MT in patients with AIS induced by anterior circulation LVO. The area under the receiver operating characteristic (ROC) curve of the predictive model was 0.813 (95% CI: 0.756-0.870), indicating a good predictive value. Conclusion The preoperative NIHSS score and PTR are independent risk factors for the prognosis of patients with AIS induced by anterior circulation LVO undergoing MT.
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Luo Xiao; Guo Fang; Qi Zhiye; Zhong Qinghua; Duan Jiang; Liang Kun
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.020
Abstract:
Objective To evaluate the effectiveness of an antibiotic stewardship quality improvement protocol in preterm infants with suspected early onset sepsis (EOS), and to assess the effects of the protocol measures on the risk of major complications in these infants. Methods A total of 446 preterm infants diagnosed with suspected EOS admitted to the Department of Neonatology, Kunming Maternal and Child Health Hospital from September 2021 to August 2023 were enrolled. An antibiotic stewardship quality improvement protocol for preterm infants with suspected EOS was developed. Infants admitted during the protocol implementation period (September 2022 to August 2023) were assigned to the experimental group (n=215), and those admitted during the preimplementation period (September 2021 to August 2022) served as the control group (n=231). Days of therapy (DOT) per 1,000 patient days (DOT/1,000 PDs), duration of antibiotic therapy, defined daily doses (DDDs), antibiotic use density (AUD), antibiotic discontinuation rates at 48 h and 72 h, and the incidence of major complications were compared between the two groups. Results Compared with the control group, the experimental group had lower DOT/1,000 PDs [181.8 (80.6, 285.7) vs. 312.5 (204.5, 555.6)], duration of antibiotic therapy [48 h (48, 72) vs. 120 h (120, 144)], DDDs [0.122 (0.098, 0.153) vs. 0.213 (0.170, 0.265)], and AUD [0.997 (0.380, 1.817) vs. 1.335 (0.745, 2.316)], with all differences being statistically significant (P<0.05). The antibiotic discontinuation rates at 48 h and 72 h in the experimental group were 70.7% and 81.9%, respectively, which were higher than those in the control group (P<0.05). Prolonged antibiotic exposure (OR=1.40, 95% CI: 1.17-1.67, P<0.001) and high DDDs level (DDDs≥0.3) (OR=4.17, 95% CI: 1.46-11.90) were independent risk factors for major complications in preterm infants with suspected EOS. Conclusion Implementation of the antibiotic stewardship quality improvement protocol reduces DOT/1,000 PDs, DDDs, AUD, and duration of antibiotic therapy, and increases antibiotic discontinuation rates at 48 h and 72 h in preterm infants with suspected early onset sepsis. Shortening antibiotic exposure time and reducing DDDs may lower the risk of major complications in these infants.
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Sang Ruizheng; Liu Bo; Liang Zhibin; Lu Chongyao; Sun Yupeng
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.021
Abstract:
Objective To investigate the association between preoperative serum microRNA-223 (miR-223) and Toll-like receptor 2 (TLR-2) levels and the occurrence of postoperative infectious complications in elderly patients with femoral neck fractures. Methods A total of 284 elderly patients with femoral neck fractures at the First Affiliated Hospital of Hebei North University from January 2022 to October 2024 were enrolled. All patients underwent proximal femoral nail antirotation (PFNA) internal fixation. Based on the occurrence of infectious complications within 28 d post-surgery, they were divided into an infection group (n=31) and a non-infection group (n=253). Propensity score matching (PSM) was performed at a 1∶1 ratio using SPSS version 28.0, with matching variables including gender, age, fracture site, fracture cause, American Society of Anesthesiologists (ASA) physical status classification, prophylactic antibiotic use, length of hospital stay, diabetes mellitus, and other general data. Preoperative serum miR-223 and TLR-2 levels were compared between the two groups. The dose-response relationship between preoperative serum miR-223 and TLR-2 levels and postoperative infectious complications was analyzed by a restricted cubic spline (RCS) model. Receiver operating characteristic (ROC) curve analysis was conducted to evaluate the value of preoperative serum miR-223 and TLR-2 levels in predicting postoperative infectious complications. The areas under the curve (AUCs) were compared using DeLong's test, and external validation was performed. Results The incidence of postoperative infectious complications within 28 d was 10.91% among the 284 elderly patients with femoral neck fractures. After PSM, no significant differences were observed in the baseline characteristics between the two groups (P>0.05). The preoperative serum C-reactive protein (CRP), procalcitonin (PCT), miR-223 and TLR-2 levels in the infection group were higher than those in the non-infection group (P<0.05). RCS analysis revealed a non-linear relationship between preoperative serum miR-223 and TLR-2 levels and postoperative infectious complications (P<0.05). The combined prediction model using both preoperative miR-223 and TLR-2 achieved an AUC of 0.893 for predicting postoperative infectious complications, which was higher than the AUCs for miR-223, TLR-2, CRP, or PCT alone (P<0.05). External validation revealed no statistically significant difference between the predicted incidence of postoperative infectious complications by the combined preoperative serum miR-223 and TLR-2 panel and the observed incidence (P>0.05). Conclusion There is a non-linear relationship between preoperative serum miR-223 and TLR-2 levels and postoperative infectious complications in elderly patients with femoral neck fractures. The combined model demonstrates high predictive efficacy, which may aid in identifying high-risk patients and guiding corresponding clinical interventions.
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Zhao Zhanfeng; Wu Xiang; Liu Jinrui; Wang Pengge; Zhang Jinghua
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.022
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Objective To investigate the expression of forkhead box Protein P3 (FOXP3) and interferon-gamma (IFN-γ) in renal transplant recipients and their relationship with the occurrence of post-transplant diabetes mellitus (PTDM). Methods A total of 190 patients who underwent renal transplantation at the 7th People's Hospital of Zhengzhou from January 2021 to August 2023 were selected. Based on the occurrence of PTDM within one year postoperatively, patients were divided into PTDM group and non-PTDM group. Clinical data, as well as FOXP3 mRNA and IFN-γ expression levels before transplantation and at 1 month postoperatively, were compared between the two groups. Receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive value of FOXP3 combined with IFN-γ for the risk of PTDM. Results Among the 190 renal transplant recipients, 44 (23.16%) developed PTDM during follow-up. The PTDM group reported higher body mass index (BMI) and proportion of perioperative rejection compared to the non-PTDM group (P<0.05). Preoperative FOXP3 mRNA level in the PTDM group was lower than that in the non-PTDM group (P<0.05), while IFN-γ levels before transplantation and at 1 month postoperatively were higher in the PTDM group than in the non-PTDM group (P<0.05). Multivariate logistic regression analysis identified BMI, preoperative FOXP3 mRNA, and preoperative IFN-γ as independent influencing factors for PTDM in renal transplant recipients (P<0.05). Based on the results of multivariate logistic regression analysis, a prediction model was established. ROC curve analysis showed that the area under the curve (AUC) of this model for predicting the risk of PTDM in renal transplant recipients was 0.920, with a sensitivity of 90.91% and a specificity of 82.19%, which were higher than those of any single indicator. Conclusion The occurrence of PTDM in renal transplant recipients is closely related to preoperative FOXP3 and IFN-γ expression levels. The regression model constructed based on these factors shows relatively high predictive value for the risk of PTDM.
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Zhang Zhihong; He Youli; Li Fang; Chen Li; Zhang Wei; Fu Yi
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.023
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Objective To investigate the relationship between preoperative neutrophil-to-lymphocyte ratio (NLR) and 90-day functional outcome after endovascular thrombectomy (EVT) in patients with severe cardioembolic stroke. Methods A total of 172 patients with severe cardioembolic stroke who underwent emergency EVT and were admitted to the intensive care unit (ICU) postoperatively at the Department of Cerebrovascular Diseases, the People's Hospital of Leshan, from January 2022 to December 2023 were enrolled. General data including gender, age, medical history, and vital signs were collected. Preoperative laboratory data including routine blood tests, blood biochemistry, and coagulation function were recorded. Additionally, information on preoperative endotracheal intubation, intravenous thrombolysis before EVT, time from onset to thrombectomy, location of thromboembolism, affected cerebral hemisphere, and intraoperative bleeding or contrast agent extravasation was documented. Receiver operating characteristic (ROC) curve analysis was conducted to analyze the predictive value of NLR for poor prognosis in patients with severe cardioembolic stroke undergoing emergency EVT. Results Among the 172 patients, 55 had a favorable prognosis and 117 had a poor prognosis, with 41 deaths (23.84%) within 90 d. Logistic regression analysis indicated that age, intraoperative bleeding or contrast agent extravasation, and NLR were independent risk factors for poor prognosis in patients with severe acute cardioembolic stroke undergoing emergency EVT (P<0.05). The area under the ROC curve (AUC) of NLR for predicting poor prognosis in these patients was 0.773, with an optimal cutoff value of 4.01. Conclusion Preoperative NLR can effectively predict the 90-day functional outcome after emergency EVT in patients with severe cardioembolic stroke. Elevated preoperative NLR is an independent risk factor for poor prognosis within 90 d after EVT in these patients, and an NLR greater than 4.01 indicates a poor prognosis.
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Li Ting; Guo Rennan; Xu Guiping
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.024
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Objective To investigate the impacts of pericapsular nerve group block (PENGB) combined with oliceridine on perioperative analgesia and recovery quality in elderly patients undergoing total hip arthroplasty (THA). Methods A total of 80 elderly patients scheduled for THA under general anesthesia at the People's Hospital of Xinjiang Uygur Autonomous Region between January and December 2024 were selected as study subjects. They were divided into an experimental group (n=40) and a control group (n=40) via a random number table. Before general anesthesia induction, the control group received intravenous sufentanil and ultrasound-guided PENGB, while the experimental group received intravenous oliceridine and ultrasound-guided PENGB. Surgery-related indicators, Visual Analog Scale (VAS) scores at various perioperative time points, recovery quality, Harris Hip Scores (HHS), and the incidence of adverse reactions were compared between the two groups. Results The experimental group showed lower resting VAS scores at 3, 6, 12, 36, and 48 h postoperatively compared to the control group (P<0.05). The total incidence of adverse reactions was 10.00% in the experimental group, which was lower than 32.50% in the control group (P<0.05). There were no significant differences in extubation time, emergence time, or post-anesthesia care unit (PACU) length of stay between the two groups (P >0.05). However, the incidence of emergence agitation was lower in the experimental group than in the control group (P<0.05). Postoperative VAS scores at all time points were lower than preoperative scores in both groups (P < 0.05). The HHS at 48 h postoperatively was higher in the experimental group than in the control group (P<0.05). Conclusion For elderly patients undergoing total hip arthroplasty under general anesthesia, compared with intravenous sufentanil, intravenous oliceridine on the basis of ultrasound-guided PENGB before induction of general anesthesia provides safe and effective perioperative analgesia. While demonstrating comparable recovery quality to sufentanil, oliceridine is associated with a reduced incidence of emergence agitation, promotes early functional recovery, and yields a lower overall rate of adverse reactions.
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2026, Doi: 10.3969/j.issn.1674-2257.2026.03.025
Abstract:
Objective To investigate the effects of the Miao ethnic herbal medicine Ardisia japonica decoction combined with auricular acupressure on the nutritional status and immune function in patients with chronic obstructive pulmonary disease (COPD). Methods A total of 110 COPD patients admitted to the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from April 2023 to April 2024 were selected as the research subjects. They were divided into a control group (auricular acupressure alone, n=55) and an experimental group (Ardisia japonica decoction combined with auricular acupressure, n=55) using a random number table method. The clinical efficacy, nutritional status, immune function, quality of life, and pulmonary function were compared between the two groups. Results The total effective rate in the experimental group (96.36%) was higher than that in the control group (83.64%) (P<0.05). After treatment, the levels of albumin (ALB), prealbumin (PAB), hemoglobin (Hb), CD3+, CD4+, the CD4+/CD8+ ratio, quality of life scores, and pulmonary function indices in the experimental group were all greater than those in the control group, while the CD8+ level was lower than that in the control group (P<0.05). Conclusion The combination of Miao ethnic herbal medicine Ardisia japonica decoction and auricular acupressure demonstrates significant efficacy for COPD patients, which improves their nutritional status, immune function, and pulmonary function, thereby enhancing their quality of life.
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Huang Yongjie; Liu Sanbao; Zhang Xuetong; Meng Zongde; Hu Shaohua; Wang Jia; Zhang Lizhu
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.026
Abstract:
Objective To evaluate the clinical efficacy of Pingxiao Capsule combined with ultrasound-guided microwave ablation (MWA) in treating benign thyroid nodules (TN). Methods A total of 120 patients with benign TN admitted to Hebei Provincial Hospital of Traditional Chinese Medicine between February 2023 and February 2024 were enrolled. They were divided into an experimental group (Pingxiao Capsule + ultrasound-guided MWA) and a control group (ultrasound-guided MWA alone) by random number table method. The maximum nodule diameter, volume reduction rate (VRR), traditional Chinese medicine (TCM) syndrome scores, clinical efficacy, thyroid hormone levels, and adverse reactions were compared between the two groups. Results At 3, 6, and 12 months post-treatment, the experimental group exhibited smaller maximum nodule diameters, lower TCM syndrome scores, and reduced thyroid-stimulating hormone (TSH) levels, alongside higher VRR, free triiodothyronine (FT3), and free thyroxine (FT4) levels compared to the control group (P<0.05). And the experimental group demonstrated a superior overall clinical efficacy rate compared to the control group (P<0.05). Conclusion The combination of Pingxiao Capsule and ultrasound-guided MWA effectively reduces nodule volume, alleviates clinical symptoms, and optimizes thyroid hormone levels in treating benign thyroid nodules.
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Zhang Jing; Su Taoli; Lu Haiyan; Wang Jing; Hou Chaohui; Cheng Yanli
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.027
Abstract:
Objective To investigate the expression levels of serum LIN28B and chitinase-3-like protein 1 (CHI3L1, also known as YKL-40) in children with acute lymphoblastic leukemia (ALL) and their correlation with prognosis. Methods A cohort of 101 children with ALL admitted to Shanxi Children's Hospital between April 2020 and January 2022 were enrolled as the ALL group. They were stratified by risk category into low-risk (n=25), intermediate-risk (n=57), and high-risk (n=19) subgroups, and divided into favorable prognosis group (n=65) and poor prognosis group (n=36). Additionally, 60 healthy children undergoing physical examinations during the same period were selected as the control group. Serum LIN28B mRNA expression levels were detected using real-time quantitative polymerase chain reaction (RT-qPCR), and YKL-40 protein expression levels were measured via enzyme-linked immunosorbent assay (ELISA). Results Serum LIN28B and YKL-40 expression levels were higher in the ALL group than in the control group (P<0.05). Serum LIN28B and YKL-40 expression levels were higher in the high-risk subgroup than in the intermediate- and low-risk subgroups, and higher in the intermediate-risk subgroup than in the low-risk subgroup (P<0.05). Serum LIN28B and YKL-40 expression levels were higher in the poor prognosis subgroup than in the favorable prognosis subgroup (P<0.05). Multivariate analysis identified risk stratification (OR=2.879), LIN28B expression level (OR=3.996), and YKL-40 expression level (OR=2.874) as independent risk factors for poor prognosis in ALL patients (P<0.05). The areas under the curve (AUCs) for serum LIN28B and YKL-40 alone in predicting poor prognosis in ALL patients were 0.810 and 0.801, respectively. The combined predictive AUC increased to 0.949, demonstrating superior predictive value compared to individual assays (Z combined vs. LIN28B =3.054, Z combined vs. YKL-40 =3.026, P<0.05). Conclusion Serum LIN28B and YKL-40 are both upregulated in children with ALL, which is closely associated with higher risk stratification and poor prognosis. Their combined detection holds high evaluation value for prognostic assessment.
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Yu Jiangtao; Zhang Qian; Li Ji; Yue Yiwei; Tian Ming
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.028
Abstract:
Objective To investigate the effects of laparoscopic dismembered pyeloplasty (transperitoneal approach) on surgical parameters, pain scores, and renal function in children with congenital severe hydronephrosis. Methods A total of 120 children with congenital severe hydronephrosis admitted to the First Affiliated Hospital of Zhengzhou University between July 2021 and March 2024 were enrolled and randomly assigned to either an experimental group (n = 60, undergoing transperitoneal laparoscopic dismembered pyeloplasty) or a control group (n=60, undergoing open pyeloplasty). Surgical parameters, postoperative pain scores, renal function, and complications were compared between the two groups. Results The experimental group demonstrated superior outcomes compared to the control group in terms of operative time (86.97±4.57 min vs 144.56±7.68 min, P<0.05), length of hospital stay (7.29±1.75 d vs 14.38±1.87 d, P<0.05), and intraoperative blood loss (12.94±4.01 mL vs 23.07±4.57 mL, P<0.05). The Visual Analog Scale (VAS) scores were lower in the experimental group than in the control group immediately after surgery (5.97±2.06 vs 8.29±2.93, P<0.05) and on the first day after surgery (3.56±1.32 vs 5.61±2.03, P < 0.05). The incidence of postoperative infections and complications in the experimental group was lower than that in the control group (6.7% vs 21.7%, P<0.05). Dismembered pyeloplasty was successfully completed in all children. At the 6-month follow-up, there were no significant differences in serum creatinine, blood urea nitrogen, cystatin C, serum uric acid, or endogenous creatinine clearance levels between the two groups (P >0.05), indicating good recovery of renal function parameters in both groups. Conclusion Transperitoneal laparoscopic dismembered pyeloplasty for treating children with congenital severe hydronephrosis can shorten operative time, effectively reduce intraoperative blood loss, decrease the incidence of postoperative infections and complications, and improve the safety of patients.
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Huang Yongfu; Xie Qiang; Luo Hao; Xie Chaofan; Wang Chunli; Li Zongcheng; Li Zishang; Shi Lei
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.029
Abstract:
Objective To investigate the impact of double-J stent indwelling time after holmium laser lithotripsy on clinical outcomes and pathogen distribution in diabetic patients with ureteral calculi (UC) and concurrent urinary tract infection (UTI). Methods A total of 116 diabetic patients with UC and concurrent UTI who underwent holmium laser lithotripsy at Ziyang Central Hospital between April 2023 and December 2024 were enrolled and randomly assigned to a control group (indwelling for 6 weeks, n=56) and an experimental group (indwelling for 4 weeks, n=60) using a random number table. The general data, laboratory indexes, pathogen distribution, postoperative stone clearance rate, and complications were collected and compared between the two groups. Results Compared with pre-insertion values, on the day of stent removal both groups showed decreased urine white blood cell (WBC) count, blood WBC count, C-reactive protein (CRP), and procalcitonin (PCT), and increased urine specific gravity (P<0.05). Compared to the control group, the experimental group demonstrated lower urine WBC count, positive rate of urine culture, blood WBC count, CRP, PCT, and positive rate of blood culture on the day of stent removal (P<0.05). Before stent insertion, 33 and 35 pathogenic strains were isolated in the control and experimental groups, respectively, of which Gram-negative bacteria accounted for 57.58% and 65.71%, predominantly Escherichia coli (E.coli), while Gram-positive bacteria accounted for 36.36% and 28.57%, predominantly Staphylococcus aureus (S.aureus), and fungi accounted for 6.06% and 5.71%. On the day of stent removal, 24 pathogenic strains were isolated in the control group: Gram-negative bacteria 58.33% (predominantly Pseudomonas aeruginosa), Gram-positive bacteria 37.50% (predominantly Enterococcus faecalis), and fungi 4.17%. In the experimental group, 11 pathogenic strains were isolated: Gram-negative bacteria 63.64% (predominantly E.coli), and Gram-positive bacteria 36.36% (predominantly S.aureus). The incidence of urinary irritation symptoms and UTI in the experimental group was lower than that in the control group (P<0.05). Conclusion After holmium laser lithotripsy in diabetic patients with ureteral calculi and concurrent urinary tract infection, a double-J stent indwelling time of ≤4 weeks effectively improves urine and blood infection-related parameters, reduces the incidence of complications such as urinary irritation symptoms and urinary tract infection, and exerts a positive impact on pathogen distribution.
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Sun Weiqing; Li Mengyun; Di Hong; Wang Wei; Ren Hongwei; Zhou Liangliang; Liu Bei
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.030
Abstract:
Objective To investigate the clinical efficacy of Jielu Qingfei Decoction combined with western medicine in the treatment of acute-phase bronchiectasis (BE). Methods A total of 160 patients with BE admitted to the Seventh People's Hospital of Hebei Province from June 2022 to June 2024 were enrolled and randomly divided into a control group (n=80) and an experimental group (n=80) using a random number table. The control group received western medicine treatment, while the experimental group received a combination of western medicine and Jielu Qingfei Decoction. The clinical efficacy, pulmonary function [forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), maximal voluntary ventilation (MVV)], blood gas analysis indicators [oxygen saturation (SaO2), partial pressure of carbon dioxide (PaCO2), partial pressure of oxygen (PaO2)], immune function, inflammatory factors [serum amyloid A (SAA), interleukin-6 (IL-6), and procalcitonin (PCT)], and the incidence of adverse reactions were compared between the two groups. Results After treatment, the total effective rate was 93.75% in the experimental group, which was higher than 81.25% in the control group (P<0.05). Compared with pre-treatment levels, after treatment both groups showed increases in FVC, FEV1, MVV, PaO2, SaO2, CD3+, CD4+, and the CD4+/CD8+ ratio, along with decreases in PaCO2, PCT, IL-6, and SAA levels (P<0.05), with the experimental group demonstrating more pronounced changes than the control group (P<0.05). There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion In the treatment of acute-phase bronchiectasis, the combination of Jielu Qingfei Decoction and western medicine can effectively reduce inflammatory factor levels, improve pulmonary function, and enhance immune system function, demonstrating definite clinical efficacy and good safety.
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Li Wei; Liu Hongxin; Chu Zhefeng; Peng Song; Zeng Linyuan
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.031
Abstract:
Objective To analyze the short- and long-term efficacy of minimally invasive locking plate internal fixation in elderly patients with Neer type Ⅱ and Ⅲ proximal humeral fractures. Methods A retrospective cohort study was conducted on 112 elderly patients with Neer type II and III proximal humeral fractures admitted to the Department of Orthopedics at Chengdu Bayi Orthopedic Hospital from January 2022 to June 2023. The patients were divided into two groups based on different surgical approaches. The control group (n=56) underwent traditional open reduction and internal fixation, while the observation group (n=56) underwent minimally invasive locking plate internal fixation. The short-term clinical efficacy was compared between the two groups at 3 months after surgery. Incision pain was evaluated using the Visual Analog Scale (VAS) on postoperative days 3, 5, and 7. After a two-year follow-up, the shoulder joint Constant-Murley score and the Barthel Index (BI) were used to assess the long-term effects of surgery on patients. Results The excellent rate in the observation group was higher than that in the control group (P<0.05). On postoperative days 3, 5, and 7, the VAS scores in the observation group were lower than those in the control group (P<0.05). At 3 months, 1 year, and 2 years after surgery, the Constant-Murley scores in the observation group were higher than those in the control group (P<0.05). At 1 and 2 years after surgery, the BI scores in the observation group were higher than those in the control group (P<0.05). Conclusion Minimally invasive locking plate internal fixation surgery can improve treatment outcomes, promote postoperative functional recovery, and improve long-term quality of life in elderly patients with Neer type Ⅱ and Ⅲ proximal humeral fractures.
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Li Xuemei; Wu Zhiqiang; Cao Xiaoying
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.032
Abstract:
Objective To explore the effects of phased pulmonary rehabilitation management on exercise capacity and quality of life in patients after lung cancer surgery. Methods A total of 64 postoperative lung cancer patients admitted between January 2024 and March 2025 were enrolled and randomly assigned to two groups (n=32 each) using a random number table. The control group received conventional intervention, while the observation group received additional phased pulmonary rehabilitation management. Both groups completed an 8-week standardized intervention. Exercise capacity, pulmonary function, cancer-related fatigue, quality of life (QoL), and satisfaction were compared between the two groups before and after the intervention. Results Before the intervention, there were no statistically significant differences in exercise capacity, pulmonary function, cancer-related fatigue levels, or quality of life between the two groups (P >0.05). After the intervention, both groups showed improvements in exercise capacity and pulmonary function, along with a reduction in cancer-related fatigue levels compared to baseline (P < 0.05). The observation group exhibited greater improvements in the 6-minute walking test (6MWT), forced expiratory volume in 1 second (FEV1), and forced vital capacity (FVC) compared to the control group, along with a lower score on the revised Piper Fatigue Scale (P<0.05). While QoL scores improved in both groups after the intervention, the intervention group showed higher scores than the control group in the emotional, lung cancer-specific, social/family, and functional domains (P<0.05). No significant difference was found in the physical domain score between the two groups (P>0.05). The observation group also reported higher satisfaction than the control group after the intervention (P<0.05). Conclusion Phased pulmonary rehabilitation management is effective in patients after lung cancer surgery. It improves exercise capacity and pulmonary function, alleviates cancer-related fatigue, enhances quality of life, and achieves higher nursing satisfaction, demonstrating high value for clinical application and promotion.
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2026, Doi: 10.3969/j.issn.1674-2257.2026.03.033
Abstract:
Objective: To explore the correlation of kinesin family member C1 (KIFC1) and spalt-like transcription factor 4 (SALL4) expression in breast cancer tissues with clinicopathological characteristics and postoperative recurrence. Methods: A total of 98 patients with breast cancer admitted to Ziyang Hospital, West China Hospital of Sichuan University (also known as Ziyang Central Hospital) between January 2022 and January 2025 were enrolled as the research objects. The expression levels of KIFC1 (57 positive cases, 41 negative cases) and SALL4 (61 positive cases, 37 negative cases) were detected in cancer tissues and adjacent non-cancerous tissues. Patients were divided into a recurrence group (n=21) and a non-recurrence group (n=77) according to whether they experienced postoperative recurrence. Differences in KIFC1 and SALL4 expression were compared among patients with different clinicopathological characteristics, and their association with postoperative recurrence was analyzed. The risk factors for postoperative recurrence of breast cancer were identified by multivariate logistic regression analysis. Results: The proportions of KIFC1 positivity (58.16%) and SALL4 positivity (62.24%) in breast cancer tissues were higher than those in adjacent non-cancerous tissues (P<0.05). No statistically significant differences were observed in age, menstrual status, maximum diameter of the tumor, tumor-node-metastasis (TNM) stage, or tumor location between patients with different KIFC1 and SALL4 expression statuses (P>0.05). Patients with positive KIFC1 and SALL4 expression had higher proportions of lymph node metastasis and HER2 positivity compared to those with negative expression (P<0.05). During follow-up (11 months to 2 years), 21 of the 98 patients experienced recurrence. The positivity rates of KIFC1 and SALL4 in the recurrence group were higher than those in the non-recurrence group (P<0.05). Multivariate logistic regression analysis identified KIFC1 positivity, SALL4 positivity, and HER2 positivity as independent risk factors for postoperative recurrence in breast cancer patients (P<0.05). Conclusion: The expressions of KIFC1 and SALL4 are up-regulated in breast cancer tissues. Positive expression of both markers is closely correlated with lymph node metastasis and HER2 positivity, and each serves as an independent risk factor for postoperative recurrence in breast cancer.
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Zhou Yulan; Shu Xiao; Yang Zijing; Yang Liangqin; Chen Qian
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.034
Abstract:
Objective: To construct a domestic intervention protocol of dignity therapy for elderly patients with end-stage disease and their family caregivers, and to explore its feasibility for clinical application in China. Methods: Through literature review and research team discussions, a preliminary version of the dignity therapy intervention protocol was drafted. The version was then revised and refined using the expert panel discussion method and a pilot trial. For the pilot trial, five family units (each comprising an elderly inpatient with end-stage disease and their primary family caregiver) were selected from the geriatric medicine center of a tertiary Grade A hospital in Chengdu, Sichuan Province, in June 2022 to examine the feasibility of the intervention protocol. Results: A total of 14 experts participated in the panel discussion, demonstrating a high expert authority coefficient of 0.89. The experts provided specific revision suggestions regarding both the dignity therapy intervention tools and the protocol itself. The revised dignity protocol, incorporating expert feedback, comprised five key themes: intervention implementers, intervention process and methods, intervention setting, quality control, and supplementary explanations. The pilot trial results showed a 100% acceptance rate of the dignity therapy intervention protocol among the five participant dyads. Conclusion: The dignity therapy intervention protocol constructed in this study is characterized by operational simplicity, high participation rates among elderly patients with end-stage disease and their family caregivers, and favorable acceptance. It demonstrates high feasibility for preliminary domestic application.
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Peng Jian; Ku Min; Huang Qiuping; Zhong Yizhu
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.035
Abstract:
Objective: To develop a questionnaire suitable for evaluating the cognitive level of nurses in the Intensive Care Unit (ICU) regarding the nursing of ventilator-induced diaphragmatic dysfunction (VIDD), and to conduct reliability and validity tests. Methods: An initial item pool for evaluating knowledge, attitudes, and practices (KAP) on VIDD care was formed through literature analysis, semi-structured interviews, and two rounds of Delphi expert consultation. After pre-survey optimization, the initial questionnaire was determined. In November 2024, 472 ICU nurses from 8 tertiary hospitals in Chongqing Municipality were selected as the research subjects using convenience sampling. Data collected were used for item screening and reliability/validity testing. Results: The final questionnaire comprised three dimensions (knowledge, attitude, and practice) with 35 items. Exploratory factor analysis indicated factor loadings for all items on their corresponding dimensions ranged from 0.604 to 0.896, and these dimensions collectively explained 72.329% of the total variance. Confirmatory factor analysis demonstrated good model fit. The average content validity index of the questionnaire was 0.947. The overall Cronbach's α coefficient of the questionnaire was 0.963, the split-half reliability was 0.791, and the test-retest reliability was 0.908. Conclusion: The knowledge, attitudes, and practices questionnaire on ventilator-induced diaphragmatic dysfunction care demonstrates good reliability and validity, and can serve as a survey tool for evaluating the cognitive level of ICU nurses regarding ventilator-induced diaphragmatic dysfunction care.
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Che Chenghang; Zhu Huan; Yao Yongping; Wang Shuang; Sun Qianqian
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.036
Abstract:
Objective: To investigate the current application status, awareness level and capacity-building needs of comprehensive geriatric assessment (CGA) among rural doctors in Western China. Methods: A questionnaire survey was conducted among 120 rural doctors in Western China who participated in August 2025 in the "Healthy Sichuan 'Health Gatekeepers' Cultivation Initiative". Their application of CGA, awareness level, and training needs were analyzed. Results: Among the surveyed rural doctors, 55.00% reported that elderly patients (≥60 years old) accounted for more than half of their total consultations; 48.33% had been exposed to CGA, yet only 6.67% were able to independently complete a full assessment, and 44.17% lacked experience in managing geriatric syndromes. The rural doctors showed a high level of recognition toward CGA but relatively low practical competence. Regarding training needs, 38.33% prioritized standardized diagnosis and treatment procedures, 50.83% preferred onsite practice, 48.33% preferred bedside teaching, 48.33% favored a practice-oriented training model, and 44.17% identified standardized clinical pathways as their most urgent requirement. Conclusion: Rural doctors in Western China exhibit insufficient CGA knowledge and limited practical experience, with an unbalanced supply-demand training structure. It is recommended to establish a practice-oriented and standardized CGA training system to improve primary geriatric care capacity.
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Wang Jiaxi; Jin Junhao; Gao Caoji; Wang Yonghua; Yang Wenli
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.037
Abstract:
Objective: To analyze the current status and influencing factors of knowledge, attitudes, and practices (KAP) regarding traumatic pain management among healthcare professionals in the emergency departments of tertiary hospitals in Chengdu. Methods: Using a convenience sampling method, 422 healthcare professionals from the emergency departments of tertiary hospitals in Chengdu were selected between May and August 2024. A self-developed questionnaire based on the KAP theory was utilized for the investigation on their general information and current status of traumatic pain management. Results: A total of 422 valid questionnaires were collected. The mean scores for knowledge, attitudes, and practices regarding traumatic pain management among emergency department healthcare professionals in tertiary hospitals in Chengdu were (44.52±8.63), (37.34±7.56), and (4.33±1.32), respectively. Multiple linear regression analysis revealed that the number of training sessions, educational background, position, and years of work experience were influencing factors for knowledge scores (P < 0.05). Educational background, number of training sessions, and professional title were influencing factors for attitude scores (P<0.05). The number of training sessions, educational background, and position were influencing factors for practice scores (P<0.05). Pearson correlation analysis showed that attitude scores of traumatic pain management were positively correlated with both practice scores (r=0.778, P<0.05) and knowledge scores (r=0.631, P<0.05), and practice scores were positively correlated with knowledge scores (r = 0.924, P < 0.05). Conclusion: Emergency department healthcare professionals in tertiary hospitals in Chengdu demonstrate positive attitudes towards traumatic pain management. However, there remains room for improvement in their knowledge levels and practical competencies.
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Wang Yangyang; Sha Sha; Yang Wenjie; Wang Ting; Wu Yumei
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.038
Abstract:
Objective: To investigate the current supply and demand of traditional Chinese medicine (TCM)-based health and elderly care services among community-dwelling older adults in Chengdu City, analyze the influencing factors, and propose optimization strategies. Methods: Using a stratified cluster random sampling method, a questionnaire survey on awareness of and demand for TCM-based health and elderly care services was conducted among 330 community-dwelling older adults selected from 11 communities randomly drawn from the urban core, inner suburbs, and outer suburbs of Chengdu City between January and March 2024. Concurrently, semi-structured interviews were conducted with 30 staff members from 11 community health service centers to assess the current status of service provision. Results: The demand rate for TCM-based health and elderly care services among respondents was 60.68%. Residing status, monthly income, type of medical insurance, level of trust in TCM, and presence of chronic diseases were independent influencing factors of demand (P<0.05). The coverage rates for basic TCM services (cupping therapy, massage, and acupuncture and moxibustion) were 100.00%; however, specialized services such as TCM dietary therapy (45.45%), and herbal footbath and medicated bath (36.36%) were insufficiently provided. Among the centers surveyed, 63.64% employed 10-20 TCM practitioners; there was a notable shortage of personnel specializing in TCM nursing, dietary guidance, and psychological care. In 90.91% of the centers, the professional titles were predominantly junior or intermediate, with practitioners holding deputy senior or higher titles accounting for ≤5%; high-level talent was severely lacking. Centers providing services with an average price ≤100 RMB accounted for 54.55%, and the pricing exhibited a discontinuous structure with no mid-range options. Conclusion: There exist structural imbalances in supply and demand, service models, and workforce allocation in community-based TCM health and elderly care services in Chengdu City. Targeted measures should be taken to optimize service provision and promote high-quality development of TCM-based health and elderly care services.
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2026, Doi: 10.3969/j.issn.1674-2257.2026.03.039
Abstract:
Osilodrostat is a new drug officially launched in China in Apr 2025, and it is the first and currently only oral medication approved domestically for the treatment of Cushing's syndrome in adults. The consensus on its clinical application varies across different countries and regions, influenced by local socio-economic contexts, disparities in healthcare resources, health insurance reimbursement policies, and standard clinical practices. This article systematically interprets and compares the differences between Chinese and American expert consensus on osilodrostat treatment for Cushing's syndrome. It analyzes dosing strategies, safety monitoring, and management of special populations, thereby providing reference and guidance for standardized drug treatment and clinical safe use in Cushing's syndrome.
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Sun Qiqi; Xu Laiyin; Li He; Sun Wencai; Wang Jun
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.040
Abstract:
Transfusion medicine is an important component of the modern clinical treatment system. Its standard operating procedures and process safety are directly related to patients' life safety and therapeutic outcomes. In recent years, with the continuous innovation of blood management technologies and in-depth integration of information technology, a full-life-cycle intelligent monitoring system centered on "one bag of blood" has been gradually established. It provides strong technical support for the full traceability of blood from collection, testing, storage to clinical transfusion[1].
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Wang Shuang; Xiao Siwen; Wen Jinhui; Li Shiyun; Wang Shan; Bai Xiujuan
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.041
Abstract:
Since the 1970s, the prevalence of overweight and obesity has been rising worldwide, and it has become a major public health issue in China. Currently, more than 50% of adults and approximately 20% of school-age children in the country are overweight or obese, and the obesity rate among adolescents in some cities even reaches as high as 40%[1]. Without effective interventions, it is projected that the prevalence of overweight and obesity in China will exceed 65% by 2030, with the related medical expenses expected to increase to 61 billion US dollars, accounting for roughly 22% of the total national medical expenditure[2].
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Li Liuying; Zhou Xia; Wang Qiuxiang; Cao Jianzhi; Feng Peimin
2026, Doi: 10.3969/j.issn.1674-2257.2026.03.042
Abstract:
Pulmonary arterial hypertension (PAH) is a malignant and progressive pulmonary vascular disease, which is mainly characterized by persistent increase of pulmonary vascular resistance, progressive pulmonary vascular remodeling and right ventricular hypertrophy. Its pathogenesis has not been fully clarified. Recent studies [1] have found that gut microbiota (GM) and its metabolites are not only essential for maintaining the integrity and function of the intestinal barrier, but may also participate in the pathological process of PAH via the "gut-lung axis".
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